Electrochemical immobilization of ascorbate oxidase in poly(3,4‐ethylenedioxythiophene)/multiwalled carbon nanotubes composite films
Identifieur interne : 000331 ( Main/Exploration ); précédent : 000330; suivant : 000332Electrochemical immobilization of ascorbate oxidase in poly(3,4‐ethylenedioxythiophene)/multiwalled carbon nanotubes composite films
Auteurs : Ming Liu [République populaire de Chine] ; Yangping Wen [République populaire de Chine] ; Dong Li [République populaire de Chine] ; Haohua He [République populaire de Chine] ; Jingkun Xu [République populaire de Chine] ; Congcong Liu [République populaire de Chine] ; Ruirui Yue [République populaire de Chine] ; Baoyang Lu [République populaire de Chine] ; Guodong Liu [République populaire de Chine]Source :
- Journal of Applied Polymer Science [ 0021-8995 ] ; 2011-10-15.
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Abstract
Immobilization of ascorbate oxidase (AO) in poly(3,4‐ethylenedioxythiophene) (PEDOT)/multiwalled carbon nanotubes (MWCNTs) composite films was achieved by one‐step electrochemical polymerization. The PEDOT/MWCNTs/AO modified electrode was fabricated by the entrapment of enzyme in conducting matrices during electrochemical polymerization. The PEDOT/MWCNTs modified electrodes were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The experimental results showed that the composite films exhibited better mechanical integrity, electrochemical activity, higher electronic and ionic conductivity, and larger redox capacitance compared with pure PEDOT films, which would be beneficial to the fabrication of PEDOT/MWCNTs/AO electrochemical biosensors. The scanning electron microscopy studies revealed that MWCNTs served as backbone for 3,4‐ethylenedioxythiophene (EDOT) electropolymerization. Furthermore, the resulting enzyme electrode could be used to determine L‐ascorbic acid successfully, which demonstrated the good bioelectrochemical catalytic activity of the immobilized AO. The results indicated that the PEDOT/MWCNTs composite are a good candidate material for the immobilization of AO in the fabrication of enzyme‐based biosensor. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
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DOI: 10.1002/app.34257
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Sci.</title><idno type="ISSN">0021-8995</idno><idno type="eISSN">1097-4628</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2011-10-15">2011-10-15</date><biblScope unit="volume">122</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="1142">1142</biblScope><biblScope unit="page" to="1151">1151</biblScope></imprint><idno type="ISSN">0021-8995</idno></series><idno type="istex">10C7A672AFFE51F88A2AFC3A2B8D3CA2C85FE623</idno><idno type="DOI">10.1002/app.34257</idno><idno type="ArticleID">APP34257</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-8995</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ascorbate oxidase</term><term>conducting polymer</term><term>electrochemical immobilization</term><term>multi‐walled carbon nanotubes</term><term>poly(3,4‐ethylenedioxythiophene)</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Immobilization of ascorbate oxidase (AO) in poly(3,4‐ethylenedioxythiophene) (PEDOT)/multiwalled carbon nanotubes (MWCNTs) composite films was achieved by one‐step electrochemical polymerization. The PEDOT/MWCNTs/AO modified electrode was fabricated by the entrapment of enzyme in conducting matrices during electrochemical polymerization. The PEDOT/MWCNTs modified electrodes were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The experimental results showed that the composite films exhibited better mechanical integrity, electrochemical activity, higher electronic and ionic conductivity, and larger redox capacitance compared with pure PEDOT films, which would be beneficial to the fabrication of PEDOT/MWCNTs/AO electrochemical biosensors. The scanning electron microscopy studies revealed that MWCNTs served as backbone for 3,4‐ethylenedioxythiophene (EDOT) electropolymerization. Furthermore, the resulting enzyme electrode could be used to determine L‐ascorbic acid successfully, which demonstrated the good bioelectrochemical catalytic activity of the immobilized AO. The results indicated that the PEDOT/MWCNTs composite are a good candidate material for the immobilization of AO in the fabrication of enzyme‐based biosensor. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Jiangxi</li></region><settlement><li>Nanchang</li></settlement></list><tree><country name="République populaire de Chine"><region name="Jiangxi"><name sortKey="Liu, Ming" sort="Liu, Ming" uniqKey="Liu M" first="Ming" last="Liu">Ming Liu</name></region><name sortKey="He, Haohua" sort="He, Haohua" uniqKey="He H" first="Haohua" last="He">Haohua He</name><name sortKey="He, Haohua" sort="He, Haohua" uniqKey="He H" first="Haohua" last="He">Haohua He</name><name sortKey="Li, Dong" sort="Li, Dong" uniqKey="Li D" first="Dong" last="Li">Dong Li</name><name sortKey="Li, Dong" sort="Li, Dong" uniqKey="Li D" first="Dong" last="Li">Dong Li</name><name sortKey="Liu, Congcong" sort="Liu, Congcong" uniqKey="Liu C" first="Congcong" last="Liu">Congcong Liu</name><name sortKey="Liu, Guodong" sort="Liu, Guodong" uniqKey="Liu G" first="Guodong" last="Liu">Guodong Liu</name><name sortKey="Liu, Ming" sort="Liu, Ming" uniqKey="Liu M" first="Ming" last="Liu">Ming Liu</name><name sortKey="Lu, Baoyang" sort="Lu, Baoyang" uniqKey="Lu B" first="Baoyang" last="Lu">Baoyang Lu</name><name sortKey="Wen, Yangping" sort="Wen, Yangping" uniqKey="Wen Y" first="Yangping" last="Wen">Yangping Wen</name><name sortKey="Wen, Yangping" sort="Wen, Yangping" uniqKey="Wen Y" first="Yangping" last="Wen">Yangping Wen</name><name sortKey="Xu, Jingkun" sort="Xu, Jingkun" uniqKey="Xu J" first="Jingkun" last="Xu">Jingkun Xu</name><name sortKey="Xu, Jingkun" sort="Xu, Jingkun" uniqKey="Xu J" first="Jingkun" last="Xu">Jingkun Xu</name><name sortKey="Yue, Ruirui" sort="Yue, Ruirui" uniqKey="Yue R" first="Ruirui" last="Yue">Ruirui Yue</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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